Coach: Jim Harbaugh
Offensive Coordinator: Greg Roman
Defensive Coordinator: Jesse Minter
Passing Game Coordinator: Marcus Brady
Senior Offensive Assistant
Stadium SoFi Stadium
Chairman: Dean Spanos
General Manager: Joe Hortiz
Training Camp: The Bolt (El Segundo, California)
| Nr. | Spieler | Pos. | College/Uni | Geburtsdatum | Jahre | Draft |
| 01 | Quentin Johnston | WR | TCU Horned Frogs | 06. Juni 2001 | 2 | Los Angeles Chargers 2023 |
| 02. | Elijah Molden | DB | Washington Huskies | 30. Januar 1999 | 4 | Tennessee Titans 2021 |
| 03 | Derwin James | SS | Florida State Seminoles | 03. August 1996 | 7 | Los Angeles Chargers 2018 |
| 04 | Taylor Heinicke | QB | Old Dominion Monarchs | 15. März 1993 | 8 | |
| 05. | Trey Lance | QB | North Dakota State Bison | 09. September 2000 | 4 | San Francisco 49ers 2021 |
| 06 | Denzel Perryman | ILB | Miami (FL) Hurricanes | 05. Dezember 1992 | 10 | San Diego Chargers 2015 |
| 07 | DJ Uiagalelei | QB | Clemson Tigers Oregon State Beavers Florida State Seminoles | 17. April 2001 | Rook | |
| 08 | Omarion Hampton | RB | North Carolina Tar Heels | 16. März 2003 | Rook | Los Angeles Chargers 2025 |
| 09 | Tre Harris | WR | Luisiana Tech Bulldogs Ole Miss Rebels | 28. Februar 2002 | Rook | Los Angeles Chargers 2025 |
| 10 | Justin Herbert | QB | Oregon Ducks | 10. März 1998 | 5 | Los Angeles Chargers 2020 |
| 11 | Cameron Dicker | K | Texas Longhorns | 06. Mai 2000 | 3 | |
| 12 | Derius Davis | WR | TCU Horned Frogs | 11. September 2000 | 2 | Los Angeles Chargers 2023 |
| 13 | Kennan Allen | WR | California Golden Bears | 27. April 1992 | 12. | San Diego Chargers 2013 |
| 15 | Ladd McConkey | WR | Georgia Bulldogs | 11. November 2001 | 1 | Los Angeles Chargers 2024 |
| 16 | JK Scott | P | Alabama Crimson Tide | 30. Oktober 1995 | 7 | Green Bay Packers 2018 |
| 20 | Cam Hart | CB | Notre Dame Fighting Irish | 12. Mai 2000 | Los Angeles Chargers 2024 | |
| 22 | Najee Harris (NON) | RB | Alabama Crimson Tide | 09. März 1998 | 4 | Pittsburgh Steelers 2021 |
| 24 | Benjamin St.-Juste | DB | Michigan Wolverines Minnesota Golden Gophers | 08. September 1997 | 4 | Washington Football Team 2021 |
| 25 | Junior Colson | LB | Michigan Wolverines | 06. Dezember 2002 | 1 | Los Angeles Chargers 2024 |
| 26 | Donte Jackson | CB | LSU Tigers | 08. November 1995 | 7 | Carolina Panthers 2018 |
| 27 | JaQuae Jackson | WT | Colifornia (PA) Vulcans Rutgers Scarlet Knight | 30. Januar 2000 | Rook | |
| 27 | R.J. Mickens | SAF | Clemson Tigers | 10. Juli 2001 | Rook | Los Angeles Chargers 2025 |
| 28 | Hassan Haskins | RB | Michigan Wolverines | 26. November 1999 | 3 | Tennesse Titans 2022 |
| 29 | Tarheeb Still | DB | Maryland Terrapins | 09. Juni 2002 | 1 | Los Angeles Chargers 2024 |
| 30 | Kimani Vidal | RB | Troy Trojans | 28. August 2001 | 1 | Los Angeles Chargers 2024 |
| 31 | Trikweze Bridges | SAF | Oregon Ducks Florida Gators | 20. September 2000 | Rook | Los Angeles Chargers 2025 |
| 31 | Nyheim HInes | RB | North Carolina State Wolfpack | 12. November 1996 | 7 | Indianapolis Colts 2018 |
| 32 | Alohi Gilman | S | Navy Midshipmen Notre Dame Fighting Irish | 17. September | Los Angeles Chargers 2020 | |
| 33 | Deane Leonard | DB | Calgary Dinos Ole Miss Rebels | 20. November 1999 | 3 | Los Angeles Chargers 2022 |
| 34 | Jaret Patterson | RB | Buffalo Bulls | 23. Dezember 1999 | 4 | |
| 35 | Jaylen Jones | S | Virginia Tech | Rook | ||
| 35 | Raheim Sanders | RB | Arkansas Razorbacks South Carolina Gamecocks | 08. Juni 2020 | Rook | |
| 36 | Ja'Sir Taylor | CB | Wake Forest Deamon Deackens | 08. Januar 1999 | 3 | Los Angeles Chargers 2022 |
| 37 | Luke Grimm | WR | Kansas | 08. August 2001 | Rrok | |
| 38 | Dalevon Campbell | WR | South Carolina | Rook | ||
| 38 | Emany Johnson | S | Nevada | !/. November 2000 | Rook | |
| 39 | Jaylen Johnson | WR | Georgia Bulldogs East Carolina Piates | 26. Mai 2000 | 1 | |
| 39 | Eric Rogers | WR | Cal Lutheran Kingsmen | 12. Februar 1991 | Rook | |
| 40 | Kendall Williamson | S | Stanford Cardinal | 24. August 2000 | 1 | Chicago Bears 2023 |
| 41 | Stevo Klotu | TE | Iowa State Cyclones | 28. Dezember 2001 | Rook | |
| 41 | Nehemiah Shelton | CB | Long Beach CC Jan Jose State Spartans | 13. September 1999 | Rook | |
| 42 | Tucker Fisk | TE | Stanford Cardinal | 19. Januar 1999 | 2 | |
| 43 | Troy Dye | OLB | Oregon Ducks | 18. September 1996 | 5 | Minesota Vikings 2020 |
| 44 | Scott Matlock | DT | Boise State Broncos | 28. Juni 2000 | 2 | Los Angeles Chargers 2023 |
| 45 | Tull Tuipulotu | DL | USC Trojans | 03. September 2002 | 2 | Los Angeles Chargers 2023 |
| 46 | Nikko Reed | CB | Colorado Buffaloes Oregon Ducks | 03. März 2003 | Rook | |
| 47 | Josh Harris | LS | Auburn Tigers | 27. April 1989 | 13 | |
| 48 | Bud Dupree | DE | Kentucky Wildcats | 12. Februar 1993 | 10 | Pittsburg Steelers 2015 |
| 49 | Myles Purchase | CB | Iowa State Cyclones | 26. November 20o2 | Rook | |
| 49 | Thomas Yassmin (Australien) | TE | Utah Utes | 21. Oktober 2000 | Rook | |
| 50 | Caleb Murphy | DE | Grand Valley State Lakers Ferris State Bulldogs | 15. Dezember 1999 | 2 | |
| 52 | Khalil Mack | OLB | Buffalo Bulls | 22. Februar 1991 | 11 | Oakland Raiders 2014 |
| 53 | Del'shawn Phillips (PUP) | OLB | Garden City CC Illinois Fighting Illini | 09. Oktorber 1996 | 5 | |
| 54 | Kyle Kennard | DE | Georgia Tech Yellow Jackets South Carolina Gamecocks | 12. Dezember 2001 | Rook | Los Angeles Chargers 2025 |
| 56 | Kana'i Mauga | LB | USC Trojans | 11. November 2001 | 1 | Los Angeles Chargers 2024 |
| 57 | Tre'mon Morris-Brash | DL | Central Florida UFC Knights | 29. September 2000 | Rook | |
| 58 | Marlowe Wax | LB | Syracuse Orange | 13. Dezember 2002 | Rook | |
| 59 | Kylan Guidry | OLB | Western Kentucky | Rook | ||
| 60 | Nesta Jade Silvera | DT | Miami (FL) Hurricanes Arizona Stade Sun Devils | 23. Januar 2000 | 2 | Las Vegas Raiders 2023 |
| 61 | Karsen Barnhart | G | Michigan Wolverines | 02. March 2001 | Rook | |
| 62 | Nash Jones | OG | Texas State | Rook | ||
| 63 | Josh Kaltenberger | C | Maryland Terrapines | Rook | ||
| 65 | Corey Stewart | T | Purdue Boilermakers | Rook | ||
| 68 | Jamaree Salyer | OL | Georgia Bulldogs | 13. Juli 2000 | 3 | Los Angeles Chargers 2022 |
| 70 | Rasawn Slater (IR) | G | Northwestern Wildcats | 26. März 1999 | 4 | Los Angeles Chargers 2021 |
| 71 | Branson Taylor | OL | Pittsburgh Panthers | 13. Februar 2002 | Rook | Los Angeles Chargers 2025 |
| 72 | David Sharpe | OT | Florida Gators | 21. Oktober 1995 | 8 | Oakland Raiders 2017 |
| 73 | Mekhi Becton | OL | Louisville Cardinals | 18. April 1999 | 5. | New York Jets 2020 |
| 74 | Ryan Nelson | OT | Virginia | 06. August 1999 | Rook | |
| 75 | Bradley Bozeman | C | Alabama Crimson Tide | 24. November 1994 | 7 | Baltimore Ravens 2018 |
| 76 | Joe Alt | OL | Notre Dame Fighting Irish | 28. Februar 2003 | 1. | Los Angeles Chargers 2024 |
| 77 | Zion Johnson | G | Davidson Wildcats Boston College Eagles | 18. November 1999 | 3 | Los Angeles Chargers 2022 |
| 78 | Andre James | T | UCLA Bruins | 02. Mai 1997 | 6 | |
| 79 | Trey Pipkins | OT | Sioux Falls Cougars | 09. Mai 1996 | 6 | Los Angeles Chargers 2019 |
| 82 | Brenden Rice | WR | Colarado Buffaloes USC Trojans | 18. März 2002 | 1 | Los Angeles Chargers 2024 |
| 83 | Tyler Conklin | TE | Central Michigan Chipewas Northwood Timberwolves | 30. Juli 1995 | 7 | Minnesota Vikings 2018 |
| 84 | KeAndre Lambert-Smith | WR | Penn State Nittany Lions Auburn Tigers | 16. Oktober 2001 | Rook | Los Angeles Chargers 2025 |
| 86 | Oronde Gadsden II | TE | Syracuse Orange | 25. Juni 2003 | Rook | Los Angeles Chargers 2025 |
| 87 | Dez Fitzpatrick | WR | Louisville Cardinals | 17. Dezember 1997 | 4 | Tenesse Titans 2021 |
| 88 | Jalen Reagor | WR | TCU Horned Frogs | 02. Januar 1999 | 5 | Philadelphia Eagles 2020 |
| 89 | Will Disley | TE | Washington Huskies | 08. Juli 1996 | 7 | Seattle Seahawks 2018 |
| 90 | Teair Tart | DT | Florida International Panthers | 28. Februar 1997 | 5 | |
| 91 | Da'Shawn Hand | DE | Alabama Crimson Tide | 14. November 1995 | 7 | Detroit Lioans 2018 |
| 92 | Justin Eboigbe | DL | Alabama Crimson Tide | 09. April 2001 | 1 | Los Angeles Chargers 2024 |
| 93 | Otito Ogbonnia | DT | UCLA Bruins | 25. August 2000 | 3 | Los Angeles Chargers 2022 |
| 94 | TeRah Edwards | DL | Illinois | Rook | ||
| 96 | Naguan Jones | DT | Michigan State Spartans | 05. Februar 1998 | 4 | |
| 97 | Garmon Randolph | OLB | Baylor Bears | Rook | ||
| 98 | Christopher Hinton | DT | Michigan Wolverines | 14. September 2000 | 3 | |
| 99 | Jamaree Caldwell | DT | Independence Pirates Houston Cougars Oregon Ducks | 30. August 2000 | Rook | Los Angeles Chargers 2025 |
| Daiyan Henley | LB | Nevada Wolf Pack Washington State Cougars | 18. November 1999 | 2 | Los Angeles Chargers 2023 |